From: Engineering bacteria to solve the Burnt Pancake Problem
BPP Plasmid constructa | Perm.b | mRFP expression on amp, IPTGc | Growth on tet, amp, IPTGd | ||
---|---|---|---|---|---|
 |  | expected | observed | expected | observed |
RR-hixC-pLac-hixC-RBS-tetA(C)-hixC | (1, 2) | - | - | + | weak |
RR-hixC-tetA(C) rev -RBS rev -hixC-pLac rev -hixC | (-2, -1) | + | - | + | + |
RR-hixC-pLac-hixC-tetA(C) rev -RBS rev -hixC | (1, -2) | - | - | - | weak |
RR-hixC-RBS-tetA(C)-hixC-pLac rev -hixC | (2, -1) | + | - | - | - |
RR-hixC-pLac rev -hixC-RBS-tetA(C)-hixC | (-1, 2) | + | + | - | + |
RR-hixC-tetA(C) rev -RBS rev -hixC-pLac-hixC | (-2, 1) | - | - | - | + |
RR-hixC-pLac rev -hixC-tetA(C) rev -RBS rev -hixC | (-1, -2) | + | + | - | - |
RR-hixC-RBS-tetA(C)-hixC-pLac-hixC | (2, 1) | - | - | - | + |