Figure 2

The prototype BioScaffold {0,5;15,10} embedded in the test circuit. Here, the internal structure of the prototype BioScaffold and the locations of the associated PsrI cut sites and recognition sequences are shown. The prototype BioScaffold (part BBa_J70399 in the Registry of Standard Biological Parts) is assembled between a promoter (BBa_R0010, which is not shown here) and GFP (BBa_E0040, which is partially shown here). The BioScaffold contains one PsrI recognition site on either side of the RFP reporter (as well as an internal PsrI site within the RFP reporter that is not shown). PsrI cuts into the scar on the left side of the part and GFP on the right side of the part, allowing the BioScaffold to be replaced with RBS sequences that control the expression of GFP. When the BioScaffold is present, its internal RFP reporter circuit is also present and should produce red fluorescent colonies. The RFP reporter circuit should be removed when the BioScaffold is removed. The cutting profile for the BioScaffold is {0,5;15,10}, using the notation {w,x;y,z} is described in the Results section of the paper. Different BioScaffolds can be created with different restriction enzyme sites, reporters, or cutting profiles.