Figure 5From: Computational design and characterization of a temperature-sensitive plasmid replicon for gram positive thermophilesPlasmid copy number vs. temperature. Panel A shows the plasmid copy number in the inoculum. Panel B shows the endpoint copy number after ~7 generations of growth at a range of temperatures from 48°C to 62°C in media without antibiotic selection. Copy number was measured by qPCR. Error bars in both panels represent one standard deviation, n = 3.Back to article page