Figure 5
From: A genetic bistable switch utilizing nonlinear protein degradation
Observing hysteresis to test for bistability. Hysteresis was measured using the PRM-GFP reporter. Blue curves represent samples cultured overnight in IPTG. Green curves represent samples culture overnight in aTc. (a) SW3 was “always on.” (b) SW4 had bistable behavior. (c) SW7 was “always off.” Each data point was measured with three biological replicates. Error bars represent the data range (highest and lowest value).