Figure 8

Transient switching behavior. Transient behavior was measured for (a) SW4 starting in the “on” state, and (b) starting in the “off” state using the PRM-GFP reporter. Transient behavior was measured for (c) starting in the “on” state, and (d) starting in the “off” state using the PLtetO-1-GFP reporter. At time t = 0 inducers are added into the culture to either reinforce the state, alter the state, or allow the state to persist (no inducer added). Each data point was measured using three biological replicates. Error bars indicate the data range (highest and lowest value). The 50% line measures the 50% point between maximum and minimum GFP expression. Because the baseline “on” and “off” GFP expression changes over time as OD increases, the 50% level also changes with OD. The 50% line is used to measure the activation and deactivation time. See methods section for more details on measuring the 50% line.