Name | BioBrick™ code | Description |
---|---|---|
pHC-attP-CS | pMK-RQ(BBa_K300983)a | DNA fragment including FRT-NheI-attP-NheI-CS(B0033)-FRT, flanked by AvrII sites, in high copy vector |
pHC-CmR | pSB1A1(BBa_P1004)b | Chloramphenicol resistance gene with promoter in high copy vector |
pHC-ter | pSB1AK3(BBa_B0015)b | Double terminator in high copy vector |
pHC-R6K | pSB1A2(BBa_J61001)b | R6K conditional replication origin in high copy vector |
pHC-CmRter | pSB1AK3(BBa_K300032)c | Chloramphenicol resistance cassette in high copy vector |
pHC-PR-RFP | pSB1A2(BBa_I763007)b | RFP constitutive expression cassette, driven by the PR promoter in high copy vector |
pHC-CmRter-R6K | pSB1A2(BBa_K300008)c | Chloramphenicol resistance cassette with R6K origin downstream in high copy vector |
pBBintФ-B0033 | BBa_K300982c | Integrative base vector, targeting the Ф80 locus, with RBS BBa_B0033 as insert |
pBBintФ-RFP | BBa_K300000(BBa_I763007)c | Integrative base vector, targeting the Ф80 locus, with RFP constitutive expression cassette as insert |
pHC-aspA | pSB2K3(BBa_C0083)b | aspA coding sequence in high copy vector |
pBBintAsp-RFP | BBa_J107058(BBa_I763007)c | Integrative vector, specialized to target the aspA locus, with RFP constitutive expression cassette as insert |
pInt80-649 | BBa_J72008d | Ampicillin-resistant low copy helper plasmid, with temperature-sensitive pSC101 origin, containing a constitutively expressed pir-116 gene and the site-specific recombination machinery of the Ф80 phage under the control of a heat-sensitive promoter. |
pCP20 | not in the Registrye | Ampicillin- and chloramphenicol-resistant low copy helper plasmid, with temperature-sensitive pSC101 origin, containing the yeast Flp recombinase under the control of a heat-sensitive promoter. |