Fig. 3

Effect of Aqp1 expression on the unfolded protein response. a, Fold changes in key UPR-associated genes, BiP, XBP1s, and CHOP in Aqp1-expressing cells relative to GFP controls. b, Fold changes in the BiP, XBP1s, and CHOP in Aqp1-expressing cells treated with 2.5 µg/mL tunicamycin for 4 h relative to vehicle-treated cells. Error bars represent the s.e.m. from n = 3 biological replicates. * P-value < 0.05, ** P-value < 0.01, *** P-value < 0.001. GAPDH and actin were used as housekeeping genes for the mouse and human cell lines, respectively