# Parametric analysis of a novel semi-circular microfluidic CD-ELISA valve

- Samuel I En Lin
^{1}Email author

**5**:15

**DOI: **10.1186/1754-1611-5-15

© En Lin; licensee BioMed Central Ltd. 2011

**Received: **28 July 2011

**Accepted: **7 November 2011

**Published: **7 November 2011

## Abstract

CD-ELISA uses the microfluidic ranking method and centrifugal force to control the testing solution as it flows into the reaction region. The most challenging part of CD-ELISA is controlling the flow process for different biological testing solutions, i.e. the controlling sequence for the microfluidic channel valves. The microfluidic channel valve is therefore the most important fluid channel structure for CD-ELISA. In this study, we propose a valve design suitable for a wide range rotational speeds which can be applied for mass production (molding). Together with supporting experiments, simulation based on two-phase flow theory is used in this study, and the feasibility of this novel valve design is confirmed. Influencing design factors for the microfluidic channel valves in CD-ELISA are investigated, including various shapes of the arc, distance d, radius r, the location of the center of the circle, and the contact angle. From both the experimental results and the simulated results, it is evident that the narrowest channel width and the contact angle are the primary factors influencing valve burst frequency. These can be used as the main controlling factors during the design.

### Keywords

CD-ELISA microfluidics valve design centrifugal force## 1. Introduction

The current method for biomedical examination tests requires a faster analyzing and determination process. Having the testing samples and testing solutions in smaller scales has become the solution. The principle of ELISA is to show the existence of a particular protein through the specificity of the bond between antigen (i.e. protein) and antibody, demonstrated by a color-change or luminescence reaction with an enzyme [1–3].

Highly specific antibody reactions and magnifying functionality have been widely used in biomedical testing, environmental analysis, and biotechnology research. Conventionally, an antibody with specificity to the expressed protein that targets foreign transformed genes is produced first during the ELISA detection process. This antibody is referred to as the first order antibody (or detection antibody). During the detection process, the body fluid protein to be detected is first placed on a membrane which prevents non-specific antibody binding. The first order antibody is added to detect and capture the specific protein. The second order antibody (or enzyme-labeled antibody) is then added. This second order antibody is linked with an enzyme and has specificity to the first order antibody. The specific linkage produced by the second order antibody and the first order antibody carries the enzyme to the location of the protein, and the enzyme substrate is added. After a period of time, the enzyme catalyzes the substrate causing reactions involving color changes or luminescence. Finally, the existence of the specific protein is confirmed if residual color changes or emissions remain at specific locations on the membrane [3, 4].

This conventional method involves excessive numbers of procedures, and each procedure requires separate execution, which significantly lengthens the detection time. CD-ELISA is an ELISA process based on a compact disk. The main principle in this process is to design and construct various microfluidic channels on a blank CD. Test solutions can be placed at specific locations first, and then the test samples can be placed in the center. The CD can then be placed in a reader, and as the CD spins, centrifugal forces are generated, creating the so-called pump. A higher rotational velocity produces a larger centrifugal force, and a lower rotational velocity produces a smaller centrifugal force. This method can be used on samples to first control the direction of flow, and then to create reactions with the testing solutions which are already placed on the CD. The reaction results can be measured via visible or UV light. The measured results are then entered into the computer for analysis.

The amount of testing solution used in CD-ELISA is five times less than that of ELISA. The usage time can also be reduced by as much as nine times [5]. The most challenging aspects of the development process for CD-ELISA are (1) the controlling of flow processes for different biological testing solutions, using microfluidic channel valves to control the sequential orders of the outflow fluid, and (2) the statistics for multi-channel sample detections, using bifurcation technology in fluidic channels to form the splitting effects in multiple microfluidic channels. There have been numerous studies involved with the design of the valve. The valve mechanism can be divided into two categories, namely active and passive. Active valves include diaphragm-type hydraulic control valves [6], surface wetting valves [7], gas bubble valves (controlled by the electrochemical method, [8]), and heat driven gels [9]. With satisfactory designs, micro-pumps can also be used as micro valves. Micro valves based on fluid contact [10], belonging to the passive category, work by placing two capillaries together; for one of them, the fluid stops as soon as it arrives, and for the other one, as the fluid arrives, the fluid changes the equilibrium based on the surface of the static fluid. Recently developed passive valves include the fishbone valve [11] and the well-type valve [12, 13]. The fishbone valve is a patented design and is already in commercial usage. However, since the fishbone design has a limited width and is susceptible to breakage, the production process is complicated. The well-type valve design considerably improves this production issue. However, the well-type valve has a small adjustable operational rotation range. When the locations of the valves are fixed, the burst frequency is almost fixed as well. In the overall microfluidic channel design or production process, if there are some errors which require modifications to the valve burst frequency, limitations apply and no modification can be performed.

In this study, we aim for a wide range rotational speed valve for practical usage and propose a novel semi-circular design structure. The effects of various design factors on the burst frequency are studied to find the obvious geometric factors. The Taguchi method is used to further study the effects of geometric factors on the rotational speed. Simulations based on two-phase flow theories are used, with the aid of practical experiments, to confirm the results.

## 2. Two-Phase Flow Theories

*ϕ*which varies between -1 and 1. The Cahn-Hilliard equations are first divided into the following equations:

where **u**, γ, λ, and *ε* represent flow velocity of the fluid, liquidity, mixing energy density, and interface thickness parameter, respectively, and *ψ* is the phase field helper variable. Generally, $\lambda =\frac{{h}_{c}}{2}$ where *h*_{
c
} depends on the size of the feature mesh on the interface, and γ = *ε*^{2}.

*η*) and the density (

*ρ*) over the mixing region are defined as:

*w*is for water and the subscript

*a*is for air. The transfer functions for momentum and mass are derived from Navier-Stokes equations, expressed as below.

*ρ*,

**u**, p,

*μ*,

**F**, and

**F**

_{ st }are the density, velocity, pressure, body force, and surface tension of the fluids, respectively. The surface tension

**F**

_{st}can be represented as

*σ*is the surface tension coefficient. At room temperature, the surface tension coefficient for water and air is approximately

*σ*= 0.0741 $\frac{N}{m}$. Supposing | is the identity matrix

**n**is the unit normal of the surface, and

**±**is the Dirac delta function, the body force (

**F**) can be represented as:

**g**,

**r**, and

*ω*represent gravity, radius, and angular velocity, respectively. In 3D simulation, since the fluid requires taking the gravity into consideration, the body force represented by vectors therefore contains one additional term, gravity

**F**

_{z}. Further representation of the velocity field is represented as:

Regarding boundary conditions, the wetted wall is defined as one of the boundary parameters and the contact angle is set to be *θ*_{
w
}= 70°. Since this is a 3D simulation, the parameter for the surrounding channel wall is applied in the same manner. Fluid is driven by rotation in this study, and therefore motion is generated from centrifugal force due to rotation. As a result, initial conditions for equation (7) are not required.

Note that, generally, angular acceleration is included as one of the entries for body force, however during the actual execution of the experiment, changes in the rotation speed (e.g. 1000 to 1500 rpm) can be achieved within two seconds, and thus the angular acceleration is neglected. The angular acceleration is also neglected during the process for solving both theories.

## 3. Simulation of valve structure parameters

Working parameters used in the two-phase flow analysis of the valve design

Parameters | values |
---|---|

Density(water) | 997 kg/m |

Dynamic viscosity(water) | 9.81 × 10 |

Density(air) | 1.293 kg/m |

Dynamic viscosity(air) | 1.71 × 10 |

Rotation (RPM) | 1300 |

Channel width and height (mm) | 0.3 by 0.3 |

Gravity | 9.8 N/m |

Surface tension coefficient | 0.0741 N/m |

Contact angle | Pi/2.4 |

Distance to center | 26 mm |

### 3.1 Analysis of the parametric design for fish-bone valves

^{th}fishbone is a function of twelve parameters: the air/liquid surface tension (z

_{1}), the contact angles of the liquid from the top view (z

_{2}), the top contact angle of the liquid from the side view (z

_{3}), the bottom contact angle of the liquid from the side view (z

_{4}), the density of the fluid (z

_{5}), the distance between the center of the CD and the beginning of the fluid in the reservoir (z

_{6}), the distance between the center of the CD and the end of the fluid flow front (z

_{7}), the width of the channel (z

_{8}), the height of the channel (z

_{9}), the width of a fishbone (z

_{10}), the distance between fishbones (z

_{11}), and the number of the fishbone (z

_{12}) within the fishbone valve. The burst frequency,

*f*

_{ bn }, can be expressed in mathematical terms [19].

_{10}, z

_{11}, and z

_{12}). In equation 14, compared with z

_{10}and z

_{11}, the parameter z

_{12}(number of the fishbone) has less influence on the burst frequency. In practical usage, z

_{12}is used to extend the time for fluid to pass through the valve, and it does not change the burst frequency [19]. We can therefore fix the value for this parameter, and discuss the other two parameters which are more influential. The model we use is a fish-bone valve structure (with z

_{12}= 5), as illustrated in Figure 2. There are two variable parameters: the width w for the valve can be varied from 50 and 100 um, and the distance q from 60 to 100 um. In this study, the depth for all of the microfluidic channels is 300 micrometers, the direction of rotation is clockwise, the rotational speed is 800 to 1000 rpm, and the simulation time is 0.1 sec, until the fluid flows out. As shown in Figure 2, as the width w changes, burst frequencies do not express any patterns, and range variation is in a small range.

### 3.2 Analysis of the parametric design for semi-circular valves

### 3.3 Parametric design for the Taguchi Method

To obtain an optimal design, we first analyse the properties of the key parameters to determine the factors and the number of levels, based on the Taguchi quality engineering method. After considering the extent of interactive effects, we then choose the appropriate orthogonal arrays to perform factor allocation and use it as a reference for system design and execution in the future. In the process of parametric design, we aim to determine the optimum factor level combination, and the selection is based on the value of the S/N ratio. In each factor, there is always one with the largest S/N ratio. This is the optimal level for this factor. Through this analysis, we can control the behavior of the factor around the region of this level. A large S/N value indicates a small change in quality, which means it is closer to the ideal goal.

*L*

_{9}(3

^{3}) are constructed as shown in Table 3. Experiments involving many factors can be done all at once. Using Table 3, each influential factor in the orthogonal table can be searched to find the effective method within a limited number of experiments.

Values of levels for each controlling factor

Geometric Parameter | R | D | S |
---|---|---|---|

Factor | A | B | C |

Leve1 1 | 0.08 | 0.06 | 0.155 |

Leve1 2 | 0.1 | 0.08 | 0.165 |

Leve1 3 | 0.12 | 0.1 | 0.175 |

*L*_{9} (3^{3})Orthogonal Arrays and the burst frequency obtained by simulation

R | D | S | RPM | |
---|---|---|---|---|

| 1 | 1 | 1 | 975 |

| 1 | 2 | 2 | 1035 |

| 1 | 3 | 3 | 978 |

| 2 | 1 | 2 | 900 |

| 2 | 2 | 3 | 890 |

| 2 | 3 | 1 | 1020 |

| 3 | 1 | 3 | 1000 |

| 3 | 2 | 1 | 1260 |

| 3 | 3 | 2 | 1090 |

*L*

_{9}(3

^{3}) in Table 3, which indicates a higher rotational speed.

For the LTB characteristic in Figure 9, it is observed that the average value is 0.61 and only A3 is greater than the average value. In Table 2, for the corresponding A3 with the value of 0.12 mm, B2 and B3 are both greater than the average value. Since B2 is greater than B3, B2 is used. In Table 2, for the corresponding B2 with a value of 0.08 mm, only C1 is greater than the average value. In Table 2, the corresponding C1 has a value of 0.155 mm.

The value of K can be calculated as *k* = *S*/*N*_{
max
} - *S/N*_{
min
} where K is the variance of the factors in the experiment, or the difference between the maximum value and the minimum value of the S/N ratio. A larger K value means a larger variance, which shows that this factor has a greater influence.

Figure 9 shows that the K value for factor A is 1.5, the K value for factor B is 0.9, and the K value for factor C is 1. Since the K value for factor A is larger, a larger radius r causes greater changes in rpm.

For the STB characteristic in Figure 10, it is observed that the average value is -60.1. A2 is smaller than the average value. In Table 2, for the corresponding A2 with a value of 0.08 mm, B1 is smaller than the average value. In Table 2, for the corresponding B1 with a value of 0.06 mm, C2 and C3 are smaller than the average value. Since C3 is smaller than C2, C3 is used. In Table 2, the corresponding C3 has a value of 0.175 mm. Figure 10 also shows that the K value for factor A is 1.5, the K value for factor B is 0.7, and the K value for factor C is 1.1. Since the K value for factor A is relatively larger comparing both LTB and STB characteristics, factor A has a greater effect on burst frequency.

## 4. Experimental verification

### 4.1 Experimental setup

Geometric parameters for four valves in the practical experiment

Model # | R(mm) | D(mm) | S(mm) |
---|---|---|---|

| 0.08 | 0.06 | 0.205 |

| 0.1 | 0.08 | 0.225 |

| 0.12 | 0.08 | 0.205 |

| 0.12 | 0.1 | 0.215 |

The CD-based microfluidic platform with a programmable stepping motor was also utilized in this experiment. The computer-controlled spindle can run at a constant rotational speed for a specified time period and move to the next specified speed. All channels were cleaned and dried using an antistatic air-gun each time in order to have uniform surface quality across all experiments. The channel surface quality (i.e. contact angle) can affect the flow velocity and therefore plays an important role in the burst frequency [15]. The general channel roughness of a molded PMMA CD ELISA is around Ra = 0.34~0.43 micrometers [15].

### 4.2 Experimental verification

## 5. Conclusions

- 1.
From the simulated results, it can be observed that using an appropriate selection of parameters, this valve design can cover 500 rpm in terms of the burst frequency range. This is approximately 5 times more than the conventional fish-bone valve (about 100 rpm). This offers a more flexible design option for the location of the valve on the CD spin disk. In addition, since the design does not contain microstructures, it is unlikely to erupt, which offers a more stable yield rate in production.

- 2.
Comparing the three parameters, r, s, and d, used in the semi-circular valve structure, when d is fixed, the narrowest width for microfluidic channel (i.e. 2 times the s-r value) is most influential on burst frequency. In terms of the geometry used in this study, we can use the microfluidic channel width to control the burst frequency when the width is smaller than 140 um. The adjustable range can be 500 rpm. For the same microfluidic channel width, the size structure of the radius can also change the burst frequency (about 200 rpm). For microfluidic channel widths greater than 140 um, the aid of surface modification is required, and the contact angle needs to be adjusted. During production control, it is particularly important to emphasize controlling the narrowest microfluidic channel width.

- 3.
In practical experiment verification, the magnitude of the contact angle, the pressure at the inlet, and the pressure at the outlet all directly affect the burst frequency. The simulated results and the experimental data agree with each other.

## Declarations

### 7. Acknowledgements

This work was partially funded by the Taiwan Ministry of Education under the superior advance program. The authors would like to thank Dr. James Lee from Ohio State University for all of his help. The authors would also like to thanks Dr. Yi-Ling Yen and Dr. L.S. Shi at National Formosa University for the CD ELISA experimental work using our provided CDs. The authors also thank students Sung-An Lin, Sung-Chi Lin for performing valve experimental work.

## Authors’ Affiliations

## References

- Madou M, Lee L, Daunert S, Lai S, Shih C:
**Design and Fabrication of CD-like Microfluidic Platforms for Diagnostics: Microfluidic Functions.***Biomedical Microdevices*2001,**3**(3):245-254. 10.1023/A:1011419515576View ArticleGoogle Scholar - Lee L, Madou M, Koelling K, Daunert S, Lai S, Koh C, Juang Y, Lu Y, Yu L:
**Design and Fabrication of CD-like Microfluidic Platforms for Diagnostics: Polymer-Based Microfabrication.***Biomedical Devices*2001,**3**(4):339-351.Google Scholar - Hongyan He, Yuan Yuan, Weixiong Wang, Nan-Rong Chiou, Epstein ArthurJ, Lee L James:
**Design and testing of a microfluidic biochip for cytokine enzyme-linked immunosorbent assay.***Biomicrofluidic*2009,**3:**022401. 10.1063/1.3116665View ArticleGoogle Scholar - Lai S, Wang S, Luo J, Lee L, Yang S, Madou M:
**Design of a Compact Disk-like Microfluidic Platform for Enzyme-Linked Immunosorbent Assay.***Anal Chem*2004,**75:**1832-1837.View ArticleGoogle Scholar - Yuan Yuan, He Hongyan, Lee L James:
**Protein A-based antibody immobilization onto polymeric microdevices for enhanced sensitivity of enzyme-linked immunosorbent assay.***Biotechnology and Bioengineering*2009,**102**(3):891-901. 15 10.1002/bit.22136View ArticleGoogle Scholar - Schomburg W, Fahrenberg J, Maas D, Rapp R:
**Active Valves and Pumps for Microfluidics.***J Micro mech Microeng*1993,**3:**216-218. 10.1088/0960-1317/3/4/012View ArticleGoogle Scholar - Cheng J, Hsiung L:
**Electro wetting(EW)-Based Valve Combined with Hydrophilic Teflon Microfluidic Guidancein Controlling Continuous Fluid Flow.***Biomedical Micro devices*2004,**6**(4):341-347.View ArticleGoogle Scholar - Suzuki H, Yoneyama R:
**Integrate M icrofluidic System with Electrochemically Activated On-Chip Pumps and Valves.***Sensors and Actuators B*2003,**96:**38-45. 10.1016/S0925-4005(03)00482-9View ArticleGoogle Scholar - Luo Q, Mutlu S, Gianchandani Y, Svec F, Fréchet J:
**Monolithic Valves for Microfluidic Chips Based on Thermo responsive Polymer Gels.***Electrophoresis*2003,**24:**3694-3702. 10.1002/elps.200305577View ArticleGoogle Scholar - Melin J, Roxhed N, Gimenez G, Griss P, Wijngaart Wvd, Stemme G:
**In a liquid-triggered liquid microvalve, The 12**^{ th }**international conference of solid state sensor, Actuator and microsystem, Boston 2003.***IEEE Boston*2003, 1562-1565.Google Scholar - Lu Chunmeng, Xie Yubing, Yang Yong, Cheng MarkMC, Koh Chee-Guan, Bai Yunling, Lee L James:
**New Valve and Bonding Designs for Microfluidic Biochips Containing Proteins.***Anal Chem*2007,**79:**994-1001. 10.1021/ac0615798View ArticleGoogle Scholar - Lin EnI:
**A novel well-type splitter design for CD ELISA application Taiwan patents TW201028686A1 and TW201041798A1.**2009.Google Scholar - Lin EnI, Ya-Fu Chuang:
*Implementation of femtosecond laser on the valve fabrication of CD-ELISA process.*IMECS 2009, HongKong; 2009.Google Scholar - Silva Gonçalo, Leal Nuno, Semiao Viriato:
**Critical pressure for capillary valves in a Lab-on-a-Disk: CFD and flow visualization, Computers and Structures.**2009.Google Scholar - Lin EnI:
**Novel bifurcation design for centrifugal microfluidic platform with wide range rotational speed.***Journal of Nanotechnology in Engineering & Medicine*2010,**2:**011001-1.View ArticleGoogle Scholar - Yue PT, Feng JJ, Liu C, Shen J:
**A diffuse-interface method for simulating two-phase flows of complex fluids.***J Fluid Mech*2004,**515:**293-317.View ArticleMathSciNetGoogle Scholar - Bruus Henrik:
**Theoretical microfluidics, MIC-Department of micro and Nanotechnology, Technical University of Denmark.**2nd edition. 2005.Google Scholar - Brenner Thilo, Glatzel Thomas, Zengerle Roland, Ducre'e Jens:
**Frequency-dependent transversal flow control in centrifugal microfluidics.***Lab chip*2005.Google Scholar - Messinger Robert:
*Microfluidics: Mathematical Modeling and Empirical Analysis of the Burst Frequencies of a Novel Fishbone Capillary Valve and the Development of an Algorithm to Calculate its Theoretical Hold Time.*Master's thesis, The Ohio State University, Department of Chemical and Biomolecular Engineering; 2006.Google Scholar

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