Figure 4

Determining if various caseins form bi-layer where first layer is bound strongly to the glass. In first set rhodamine labeled casein was incubated in flow cell, washed with antifade solution and the fluorescent intensity was measured using total internal reflection microscopy (TIRF). In the second set, unlabeled casein was incubated before injecting in the labeled casein. The flowcell was washed with antifade solution and fluorescence was measured using TIRF.