Figure 5

Fluorescent intensity (arbitrary units) of rhodamine labeled kinesin as measured using total internal reflection microscopy. The flow cell was incubated for 10 minutes with whole casein or different casein subunit solutions. Rhodamine labeled kinesin was then injected into the flow cell and the fluorescence was measured using total internal reflection microscopy (TIRF). Finally, the flow cells were injected with antifade solution and fluorescence was again measured by TIRF.