Table 1 Endonuclease sites targeted for removal from BioBrick vector parts.
Endonuclease | Description |
|---|---|
EcoRI, XbaI, SpeI, PstI | BioBrick restriction site |
ApoI, MfeI | Produces compatible ends to EcoRI |
AvrII, NheI | Produces compatible ends to XbaI and SpeI |
NsiI SbfI | Produces compatible ends to PstI |
AarI, AcuI, BbsI, BciVI, BfuAI, BmrI, BsaI, BsgI, BsmBI, BsmI, BspMI, BsrDI, BtgZI, EarI, EcoP15I, FokI, SapI, TspRI | Offset cutter |
I-CeuI, I-SceI, PI-PspI, PI-SceI, I-PpoI | Homing endonuclease |
Nt.BbvCI, Nt.BstNBI, Nt.AlwI | Nicking endonuclease |
AgeI, AscI, BamHI, BbvCI, FseI, HindIII, KasI, NcoI, NdeI, NgoMIV, PacI, PmeI (MssI), RsrII, SacI, SalI, SfiI, SgfI, SgrAI, SrfI, SwaI (SmiI), XcmI, XhoI, XmaI, XmnI, ZraI | Restriction endonuclease |