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Table 1 Endonuclease sites targeted for removal from BioBrick vector parts.

From: Engineering BioBrick vectors from BioBrick parts

Endonuclease Description
EcoRI, XbaI, SpeI, PstI BioBrick restriction site
ApoI, MfeI Produces compatible ends to EcoRI
AvrII, NheI Produces compatible ends to XbaI and SpeI
NsiI SbfI Produces compatible ends to PstI
AarI, AcuI, BbsI, BciVI, BfuAI, BmrI, BsaI, BsgI, BsmBI, BsmI, BspMI, BsrDI, BtgZI, EarI, EcoP15I, FokI, SapI, TspRI Offset cutter
I-CeuI, I-SceI, PI-PspI, PI-SceI, I-PpoI Homing endonuclease
Nt.BbvCI, Nt.BstNBI, Nt.AlwI Nicking endonuclease
AgeI, AscI, BamHI, BbvCI, FseI, HindIII, KasI, NcoI, NdeI, NgoMIV, PacI, PmeI (MssI), RsrII, SacI, SalI, SfiI, SgfI, SgrAI, SrfI, SwaI (SmiI), XcmI, XhoI, XmaI, XmnI, ZraI Restriction endonuclease
  1. A list of endonuclease sites targeted for removal from BioBrick vectors parts. The endonuclease sites were targeted for removal to enable various end-user DNA cloning and manipulation applications.