Engineering BioBrick vectors from BioBrick parts

Table 2 Numeric abbreviations for plasmid replication origins in BioBrick vector nomenclature.

Number	Replication origin	Copy number	Purpose
1	modified pMB1 derived from pUC19	500–700	Easy plasmid DNA purification
2	F and P1 lytic derived from pSCANS-1-BNL [67]	1–2 inducible to high copy	Inducible copy number
3	p15A derived from pMR101	10–12	Multi-plasmid engineered systems
4	rep101, repA derived from pSC101	5	Small cell to cell copy number variation
5	derived from F plasmid	1–2	Improved plasmid stability
6	pMB1 derived from pBR322	15–20	Multi-plasmid engineered systems

BioBrick vector names take the form pSB#X#. The first number indicates the identity of the origin of replication. The number, corresponding replication origin, expected plasmid copy number and typical purpose of that origin are listed [38]. To expand the list to include additional replication origins, document additions at the Registry of Standard Biological Parts [66].

ISSN: 1754-1611