Engineering BioBrick vectors from BioBrick parts

Table 4 Numeric abbreviations for vector version number in BioBrick vector nomenclature.

Number	Key features	Purpose	Example	Designer
0	absent or incomplete BioBrick cloning site		pSB2K0	Brookhaven National Lab
1	complete BioBrick cloning site (BCS)	assembly of BioBrick parts	pSB4A1	Reshma Shetty
2	5' terminator and BCS	transcriptional insulation of vector upstream of cloned BioBrick part	pSB1A2	Tom Knight
3	5' terminator and BCS and 3' terminator	transcriptional insulation of vector downstream of cloned BioBrick part	pSB1AC3	Reshma Shetty & Tom Knight
4	pSB2K3-derived vector free of many restriction sites	Genome refactoring [68]	pSB2K4	Leon Chan
5	constructed from BioBrick base vector	standardized BioBrick vector design	pSB4K5	Reshma Shetty
6	Reserved	-	-	-
7	BCS flanked by terminator BBa_B0015	transcriptional insulation of cloned BioBrick part	pSB1A7	Karmella Haynes
8	Unassigned	-	-	-
9	Unassigned	-	-	-
10	Screening plasmid v1.0 [69]	characterization of single input, single output transcriptional devices	pSB1A10	Josh Michener & Jason Kelly

BioBrick vector names take the form pSB#X#. The second number indicates the BioBrick vector version number. The version number, key features, purpose for which that version was designed, example vector, and vector designer(s) are listed. To expand the list to include new vector version numbers, document additions at the Registry of Standard Biological Parts [66].

ISSN: 1754-1611