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Table 1 Two-pancake permutations and their phenotypes

From: Engineering bacteria to solve the Burnt Pancake Problem

BPP Plasmid constructa Perm.b mRFP expression on amp, IPTGc Growth on tet, amp, IPTGd
   expected observed expected observed
RR-hixC-pLac-hixC-RBS-tetA(C)-hixC (1, 2) - - + weak
RR-hixC-tetA(C) rev -RBS rev -hixC-pLac rev -hixC (-2, -1) + - + +
RR-hixC-pLac-hixC-tetA(C) rev -RBS rev -hixC (1, -2) - - - weak
RR-hixC-RBS-tetA(C)-hixC-pLac rev -hixC (2, -1) + - - -
RR-hixC-pLac rev -hixC-RBS-tetA(C)-hixC (-1, 2) + + - +
RR-hixC-tetA(C) rev -RBS rev -hixC-pLac-hixC (-2, 1) - - - +
RR-hixC-pLac rev -hixC-tetA(C) rev -RBS rev -hixC (-1, -2) + + - -
RR-hixC-RBS-tetA(C)-hixC-pLac-hixC (2, 1) - - - +
  1. a Two-pancake BPP constructs in plasmid pSB4A3.
  2. b Signed permutation corresponding to each BPP construct.
  3. c Cells carrying a BPP plasmid were selected on ampicillin (amp) plates. IPTG-induced expression of mRFP was visually detected as fluorescent red color (+).
  4. d Serial dilutions of colonies showed normal (+), weak, or no (-) growth on tetracycline (tet) upon IPTG-induced tetA(C) expression.