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Table 1 Two-pancake permutations and their phenotypes

From: Engineering bacteria to solve the Burnt Pancake Problem

BPP Plasmid constructa

Perm.b

mRFP expression on amp, IPTGc

Growth on tet, amp, IPTGd

  

expected

observed

expected

observed

RR-hixC-pLac-hixC-RBS-tetA(C)-hixC

(1, 2)

-

-

+

weak

RR-hixC-tetA(C) rev -RBS rev -hixC-pLac rev -hixC

(-2, -1)

+

-

+

+

RR-hixC-pLac-hixC-tetA(C) rev -RBS rev -hixC

(1, -2)

-

-

-

weak

RR-hixC-RBS-tetA(C)-hixC-pLac rev -hixC

(2, -1)

+

-

-

-

RR-hixC-pLac rev -hixC-RBS-tetA(C)-hixC

(-1, 2)

+

+

-

+

RR-hixC-tetA(C) rev -RBS rev -hixC-pLac-hixC

(-2, 1)

-

-

-

+

RR-hixC-pLac rev -hixC-tetA(C) rev -RBS rev -hixC

(-1, -2)

+

+

-

-

RR-hixC-RBS-tetA(C)-hixC-pLac-hixC

(2, 1)

-

-

-

+

  1. a Two-pancake BPP constructs in plasmid pSB4A3.
  2. b Signed permutation corresponding to each BPP construct.
  3. c Cells carrying a BPP plasmid were selected on ampicillin (amp) plates. IPTG-induced expression of mRFP was visually detected as fluorescent red color (+).
  4. d Serial dilutions of colonies showed normal (+), weak, or no (-) growth on tetracycline (tet) upon IPTG-induced tetA(C) expression.
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Journal of Biological Engineering

ISSN: 1754-1611