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Figure 5 | Journal of Biological Engineering

Figure 5

From: Synthetic control of a fitness tradeoff in yeast nitrogen metabolism

Figure 5

Synthetic control of Dal80p allows for tuning of environment-dependent fitness. (A) The fitness of the engineered strain displays varying trends with ammonia concentrations at different Dal80p expression levels. Dal80p expression levels were varied by altering the concentration of galactose in the media. Fitness was measured at the indicated ammonia concentrations using the competition fitness assays and is reported as in Figure 3d. (B) The fitness of the engineered strain does not change with varying concentrations of other nitrogen sources at different Dal80p expression levels. Dal80p expression levels were varied by altering the concentration of galactose in the media. Fitness was measured at the indicated ammonia concentrations using the competition fitness assays and is reported as in Figure 3d. (C) Relative fitness in high and low ammonia concentrations is tuned through the exogenous addition of galactose to the engineered strain. Relative fitness (Wenv) is reported as the ratio of fitness in 556 mM ammonia to fitness in 8.7 mM ammonia using the competition fitness assays. Low galactose concentrations tune the strain to exhibit greater fitness in ammonia-rich conditions than ammonia-poor conditions (Wenv > 1), whereas high galactose concentrations tune the strain to exhibit greater fitness in ammonia-poor conditions that ammonia-rich conditions (Wenv < 1).

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