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Figure 1 | Journal of Biological Engineering

Figure 1

From: A switchable light-input, light-output system modelled and constructed in yeast

Figure 1

Light-responsive gene promoter system. Target gene expression is activated by red light, when photoactivated phytochrome A (Pfr_GBD) interacts with PFL_GAD and recruits it to the target promoter with Gal UAS sites for transcription activation. Expression of the gene could be switched off by far red application, when photoinactivated phytochrome (PR_GBD) dissociates from FHL_GAD. (A) Schematic representation. (B) SBGN representation [21]. Numbers correspond to following reactions: 1 Phytochrome Pr-Pfr photoconversion by red light (R). 2 Phytochrome Pfr-Pr photoconversion by far red light (FR). 3 Pfr-Pr dark reversion. 4 Pfr dissociation from SAP (Sequestrated Areas of Phytochrome). 5 Pr association to SAP. 6 Phytochrome (Pfr_GBD) association with FHL_GAD. 7 Pfr_FHL-Pr_FHL transition by FR 8 Intermediate Pr_FHL complex dissociation. 9 Transcription of luciferase gene. 10 Translation of luciferase mRNA. 11 Luciferin- luciferase enzymatic reaction

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