Figure 6

Time course of luciferase luminescence intensity in different light conditions. Experiment results (markers) and simulation (solid lines) are presented at the same scale. (A) Yeast cells containing PhyA_GBD and FHL_GAD were incubated in the dark. Luciferin was added at time zero with application of: no pulse (dark),10 min red pulse (RL), or 10 min red pulse followed immediately by 10 min far red pulse (FRL) at 18 h. (B) On-Off experiment with different time intervals between red and far red pulses. Yeast cells containing both PHYA-GBD andd FHL-GAD were preincubated with PCB in the dark. Luciferin was added at time zero. At time 18 h all the cultures were given the 10 min red pulse (RL) or 10 min red pulse with subsequent 10 min far- red pulse at 0 (RL-FRL), 0.5 (RL-0.5 h-FRL), 1 (RL-1 h-FRL), 3 (RL-3 h-FRL) and 9 h (RL-9 h-FRL) after red pulse. A representative dataset of 5 replicates is shown.