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Figure 8 | Journal of Biological Engineering

Figure 8

From: A switchable light-input, light-output system modelled and constructed in yeast

Figure 8

Model simulation and predictions. (A) Single peaks. Long term On-Off model simulation with far red light application in different time intervals after red pulse. Luciferin was added at time zero. At time 18 h all the cultures were given the 10 min red pulse or the 10 min red pulse with a subsequent 10 min far-red pulse at 0, 0.5, 1, 3 and 9 h after the red pulse. (B) Multiple peaks. Model simulation with different time intervals between red and far red pulses. The green curve corresponds to 10 h between RL pulses and 5 h between RL and FRL pulses, the black curve to 24 h between RL pulses and 5 h between RL and FRL pulses, the blue curve to 10 h between RL pulses and 2 min between RL and FRK pulses, and the red curve to 24 h between RL pulses and 2 min between RL and FRL pulses. (C) Possible profile of transcription activation with RL pulse at time 18 h and subsequent RL-FRL pulses with 2 h between RL and 2 min between RL-FRL until time 133 h-"square" shape. (D) Possible profile of transcription activation with RL and FRL pulses of different frequencies of RL (RL-FRL is 2 min everywhere):5 h-24 h-5 h-"sigma" shape

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