A switchable light-input, light-output system modelled and constructed in yeast

Table 1 Final parameter set for PhyA-FHL model

Parameter description	Parameter name	Parameter value	Dimensions	Literature data
Total PhyA concentration	Pool_PhyA	4.93E+01	nM
Total FHL concentration	Pool_FHL	1.00E+02	nM
Rate of assocoation for PhyA and FHL	K1	1.50E+00	1/nM*h
Rate of basic (background) transcription for GAL4 promoter	k_base	4.50E-01	nM/h
Rate transition to free pool	K2	1.00E-02	1/h
Rate of sequastration to SAPs	K3	9.90E+00	1/h
Rate of Pr_FHL complex dissociation	K_dis	3.00E-02	1/nM*h
Rate of dark reversion	K_rev	2.00E-01	1/h	0.027-1.2 [14, 35]
Rate of photoactivation by R light	Ka_R	4.96E-03	m2/um	4.96E-03 [30]
Rate of photoactivation by FR light	Ka_FR	3.55E-05	m2/um	3.55E-05 [30]
Rate of photoinactivation by R light	Ki_R	7.44E-04	m2/um	7.44E-04 [30]
Rate of photoinactivation by FR light	Ki_FR	1.70E-03	m2/um	1.70E-03 [30]
Rate of luciferase RNA degradatin	m_luc	3 (12 min)	1/h
Rate of luciferase translation	p_luc	1.00E+01	1/h*nM
Rate of luciferase protein degradation	m_LUC	0.8 (48 min)	1/h	0.23-1.3 [28, 29]
Rate of the luciferin-dependent luciferase degradation	m_LUC1	0.00E+00	1/h
Luciferase activity constant	k_kat	4.99E+02	nM/h	144 [28]
Michaelis constant for luciferin	Km	1.90E+00	mM	0.1-2 [28]
Hill coefficient for transcription	a_luc	2.00E+00
Maximum transcription rate	n_luc	2.94E+01	nM/h
Light conversin factor	RLU	1.00E+01
Michaelis constant for transcription	g_luc	1.04E+00	nM

ISSN: 1754-1611