Figure 2
Effect of BSA on Aβ 1–40 fibril growth via monomer addition. Aβ1–40 fibril in 40 mM Tris-HCl (pH 8.0) containing 10 μM thioflavin T was pre-incubated for 15 min alone or in the presence of BSA. Solutions were then diluted for final concentrations of 2 μM Aβ1–40 fibril (concentration expressed in monomer units) with 0 μM (control, [white circle]), 0.5 μM [black square], 2 μM [black triangle], or 10 μM [black diamond] BSA, and 40 μM Aβ1–40 monomer was added to induce fibril growth. Incorporation of Aβ1–40 monomer into fibrillar structures was monitored using thioflavin T fluorescence. Linear regression (solid lines) was performed to determine growth rates. Results are representative of three independent experiments.