Figure 3
Effect of BSA inhibition of Aβ 1–40 monomer aggregation on endothelial adhesion in HBMVECs. HBMVEC monolayers grown to confluence in 96-well plates were stimulated via 24 h incubation. Confluent monolayers were incubated alone (-CONT) or with 5 μM Aβ1–40 aggregates formed in the absence (+CONT) or presence of BSA at concentrations 2-fold (BSA, 2X) or 4-fold (BSA, 4X) in excess of Aβ1–40 monomer. Activated monolayers were washed and adhesion of Calcein-labelled THP-1 cells (2 × 104 cells/well) was assessed via fluorescence as described in Materials and Methods. * p < 0.05, ** p < 0.01, *** p < 0.001. Results are representative of three independent experiments performed with six repetitions. Error bars represent SEM.