Table 1 Operation conditions for separation of particles of different size by FlFF
Operating conditions for particles 10–250 nm | ||||||
|---|---|---|---|---|---|---|
Focus step | Elution time min | Flow rate Tip ml/min | Flow rate Focus ml/min | Flow rate Cross ml/min | Flow rate Detector ml/min | Flow rate Slot ml/min |
Elution step | NA | 0.1 | 3.9 | 1 | 0.4 | 2.6 |
   Stage 1 | 7 | 4 | NA | 1 | 0.4 | 2.6 |
   Stage 2 | 5 | 3.7 | NA | 0.7 | 0.4 | 2.6 |
   Stage 3 | 5 | 3.7 | NA | 0.7 | 0.4 | 2.6 |
Rinse step | 5 | 3 | 0 | 0 | 0.4 | 2.6 |
Operating conditions for particles less than 10 nm | ||||||
Focus step | Elution time min | Flow rate Tip ml/min | Flow rate Focus ml/min | Flow rate Cross ml/min | Flow rate Detector ml/min | Flow rate Slot ml/min |
Elution step | NA | 0.1 | 4.4 | 3.5 | 0.4 | 0.6 |
   Stage 1 | 12 | 4.5 | NA | 3.5 | 0.4 | 0.6 |
   Stage 2 | 0.5 | 3.0 | NA | 2 | 0.4 | 0.6 |
   Stage 3 | 0.5 | 2.0 | NA | 1 | 0.4 | 0.6 |
Rinse step | 8 | 3 | 0 | 0 | 0.2 | 2.8 |