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Table 1 Core definitions of the BglBrick standard

From: BglBricks: A flexible standard for biological part assembly

BglBrick plasmid

A BglBrick vector plus a BglBrick part, where the part can be either basic or composite. The nomenclature for a BglBrick plasmid is "vectorName-partName" and its sequence is the concatenation of its vector and part(s) sequences.

BglBrick part

A DNA sequence flanked by "GATCT" on the 5' end and by "G" on the 3' end, and lacking BamHI, BglII, EcoRI, and XhoI restriction sites.

BglBrick vector

A DNA sequence flanked by "GATCC" on its 5' end and by "A" on its 3' end.

BglBrick entry vector

A special type of vector sequence containing a unique EcoRI site, no BamHI or BglII restriction sites, and at most one XhoI site 5' to the EcoRI site.