Figure 1

Desired BioBrick circuit modifications and approach with BioScaffolds. Every BioBrick standard biological part (a) consists of a DNA sequence embedded between a "prefix" sequence (purple box) and a "suffix" sequence (yellow box). Parts may also contain scars (b), which form when two parts, such as "A1" and "A2" in (c) are fused together using BioBrick assembly [12]. In many cases one would like to convert an undesired scar between two parts in a BioBrick assembly into a different part or completely remove it (d). Our approach is to create a new BioBrick part (the BioScaffold) (e). The BioScaffold can be assembled into a circuit using BioBrick assembly, but unlike normal BioBricks it can be removed and replaced with a new part (f). In this paper we develop a single prototype BioScaffold that illustrates how BioScaffolds can be used to either insert parts or remove scars.