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Table 1 Sequences generated by the RBS Calculator

From: Introduction of customized inserts for streamlined assembly and optimization of BioBrick synthetic genetic circuits

Target

Rate

Sequence

10

10.5

CTAAATAGGAGGCTGGGAGTTCAACGAAACCCCT

100

95.6

CCCCCGTTCACTATACCGCAGGCCTTCTTTACAAA

1,000

950.1

ACATTAACCTACAAAGAACGTCGCAGAGGGA

10,000

9,953.7

TGTCGCGGATACTGATCCATAAAGGCCGGGGTT

100,000

94,459.0

TAGAGCCGTTAAAGAAGCTAGGAGGCCGAA

  1. 10, 100, 1,000, 10,000, and 100,000 were entered as target initiation rates in the RBS calculator. The RBS calculator uses a relative scale to relate relative translation initiation rate with five terms that quantify the strengths of molecular interactions involved in this process, with the result that any RBS sequence designed by the RBS Calculator can be related on this scale (further descriptions of this unitless parameter are available in the document [36] and FAQs [77, 78] describing this software. Qualitatively from visual analysis of GFP expression in our samples and quantitatively based on our FACS results, we consider 10-100 to be low rates of translation initiation, 1,000 to be a medium rate, and 10,000 and above to be high rates of translation initiation.
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Journal of Biological Engineering

ISSN: 1754-1611