Figure 7

Live/dead cell staining and the quantification of cell survival. (A) Representative fluorescent images of PC 12 cells, approximately 15 minutes after acrolein challenge (100 μM). Cells were exposed to Chi-TPP/Hy and Chi-DS/Hy, respectively. (B) PC 12 cells survival rate was assessed as a ratio of live cells to total cells. Consistent with previous assays, survival was significantly increased as a result of the application of both types of chitosan nanoparticles. Results are expressed as percent control values ± SD (n = 5).