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Figure 1 | Journal of Biological Engineering

Figure 1

From: Insulation of a synthetic hydrogen metabolism circuit in bacteria

Figure 1

Overview of synthetic pathway design and insulation strategies A.) Natural and synthetic pyruvate metabolism to acetyl-CoA in E. coli through the pyruvate dehydrogenase complex (PDH), pyruvate formate lyase (PFL), and the heterologous PFOR-ferredoxin (Fd)-hydrogenase synthetic pathway. Native enzymes are indicated in black, heterologous enzymes in blue.~~~B.) Insulation strategies for synthetic electron transfer pathways; deletion of competing reactions, optimization of binding surfaces, direct protein-protein fusion, and localization to a synthetic protein scaffold. We present the maximum fold increase in hydrogen production due to each method, calculated by comparing normalized values of hydrogen production by otherwise identical synthetic pathways with and without the insulation strategy (see Results).

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