Figure 5

Increase in hydrogen production by hydrogenase-ferredoxin fusion A.) Schematic model of the protein fusion, here showing the C. acetobutylicum hydrogenase fused to the spinach ferredoxin N-terminus (N-termini highlighted in blue, C-termini in green, and iron-sulfur clusters in orange). B.) Hydrogenase-ferredoxin fusion proteins are highly expressed and are the predicted size for the chimera, as indicated by western blotting with an anti-ferredoxin antibody. C.) Linker-length dependent behavior of fusion with spinach ferredoxin to hydrogenase C-terminus in vivo or in vitro. D.) Linker-length dependent behavior of fusion with Clostridium ferredoxin at the hydrogenase N- or C-terminus in vivo.