Figure 2
From: Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70
Protein synthesis as a function of plasmid concentration. (A) Luc synthesized as a function of plasmid pBEST-UTR1-Luc concentration in 3-PGA buffer (conditions: 0 mM magnesium glutamate, 80 mM potassium glutamate, 1.5 mM of each amino acids, 0.5% PEG 8000). (B) Kinetics of eGFP expression as a function of plasmid pBEST-UTR1-eGFP concentration in 3-PGA buffer (conditions: 4 mM magnesium glutamate, 60 mM potassium glutamate, 1 mM of each amino acids, 2.5% PEG 8000). Concentrations of magnesium glutamate and potassium glutamate reported here are the concentrations added to the cell-free reaction. The crude extract, dialyzed against the S30 buffer B, brings an additional 4.5 mM magnesium glutamate and 20 mM potassium glutamate to the cell-free reaction.