Skip to main content
Figure 1 | Journal of Biological Engineering

Figure 1

From: Study of messenger RNA inactivation and protein degradation in an Escherichia coli cell-free expression system

Figure 1

Maturation time of Luc and deGFP in a cell-free system. RNase A was added to cell-free reactions after 30 minutes of incubation to stop transcription and translation. (A) Kinetics of Luc expression (1 nM plasmid pBEST-UTR1-Luc) with and without addition of RNase A. (B) Kinetics of deGFP expression (5 nM plasmid pBEST-OR2-OR1-Pr-UTR1-deGFP-T500) after addition of RNase A. Inset: kinetics of deGFP expression (5 nM plasmid pBEST-OR2-OR1-Pr-UTR1-deGFP-T500) with and without addition of RNase A on a larger time scale.

Back to article page