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Figure 2 | Journal of Biological Engineering

Figure 2

From: Study of messenger RNA inactivation and protein degradation in an Escherichia coli cell-free expression system

Figure 2

deGFP messenger RNA lifetime in a cell-free reaction. The endogenous mRNA inactivation rate was measured with the reporter deGFP in an assay based on Tagetin, a RNA polymerase inhibitor. (A) Kinetics of deGFP expression (5 nM pBEST-OR2-OR1-Pr-UTR1-deGFP-T500) as a function of Tagetin concentration. Tagetin was added at the beginning of reaction. (B) Kinetics of deGFP expression (5 nM pBEST-OR2-OR1-Pr-UTR1-deGFP-T500) after addition of Tagetin (30 μM). Tagetin was added to the cell-free reactions after 30 and 45 minutes of incubation.

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Journal of Biological Engineering

ISSN: 1754-1611