Figure 2

DMJ does not reduce SVGmu production or display on vector-producing cells. 293T cells were transiently transfected by the lentiviral backbone vector encoding the GFP gene (FUGW), packaging constructs (REV and RRE), and a plasmid encoding either SVGmu or VSVG. SVGmu-staining and flow cytometry analysis of the cells two days post-transfection revealed that cells cultured with or without DMJ exhibited similar levels of SVGmu and GFP, indicating that the presence of DMJ did not restrict either glycoprotein or LV production. Control cells transfected by VSVG were similarly unaffected by DMJ and SVGmu-negative, as expected.