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Figure 4 | Journal of Biological Engineering

Figure 4

From: Production of lentiviral vectors with enhanced efficiency to target dendritic cells by attenuating mannosidase activity of mammalian cells

Figure 4

LVs produced in DMJ contain more high-mannose structures. Vectors were produced with or without DMJ, concentrated, and digested by EndoH, an enzyme that cleaves high-mannose structures. A western blot analysis of the undigested and digested vectors showed that the vectors produced in DMJ had a lower molecular weight after EndoH digestion compared to that of the vectors produced without DMJ. These results infer that the vector produced with DMJ had more high-mannose structures than the vector produced without DMJ.

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Journal of Biological Engineering

ISSN: 1754-1611