Skip to main content
Figure 5 | Journal of Biological Engineering

Figure 5

From: Production of lentiviral vectors with enhanced efficiency to target dendritic cells by attenuating mannosidase activity of mammalian cells

Figure 5

LVs produced in DMJ bind more readily to human DCSIGN than vectors produced without DMJ. (A) Vectors produced with or without DMJ were incubated with fixed 293T.DCSIGN cells, a cell line that stably displays human DCSIGN. Flow cytometric analysis of SVGmu-stained cells revealed that the vectors produced in DMJ bound to the DCSIGN-expressing cells over 6 times more readily than the vectors produced without DMJ. (B) Vectors were labeled with [35S]-Trans and incubated with either 293T or 293T.DCSIGN cells. A mannose inhibition assay was also included to determine the dependency of vector-receptor binding on the mannose-rich structures on the vector. Radioactivity analysis revealed that the vector produced with DMJ bound much more readily to the 293T.DCSIGN cells than to the 293T cells, while the addition of mannose greatly reduced cell-vector binding. Vectors produced without DMJ did not exhibit as much of a difference between binding to the 293T.DCSIGN cells and the 293T cells, while the presence of mannose reduced vector levels in both of the cell lines.

Back to article page