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Figure 4 | Journal of Biological Engineering

Figure 4

From: Rapid optimization of gene dosage in E. coli using DIAL strains

Figure 4

Optimization of violacein production. Variants of JTK160 (repA) or JTK164 (pir) were transformed with plasmids containing either a weak promoter (pBjk2741-jtk3070 or pBjk2807-jtk3070) or strong promoter (pBjk2741-jtk3080 or pBjk2807-jtk3080) driving expression of the violacein synthesis pathway. Transformants were plated as 5 μl spots. Small circles for a given strain set/plasmid combination are cropped from the same original plate image taken on a flatbed scanner. Gradient indicates the trend of copy number in strain variants.

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