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Figure 5 | Journal of Biological Engineering

Figure 5

From: Rapid optimization of gene dosage in E. coli using DIAL strains

Figure 5

Schematic of templates constructed for genomic integration. Plasmids pBjk2648r-jtk2951 and pBjk2741-jtk3041 both contain: 1) a ~500 bp homology arm (HA) matching the 5' end of the genomic insertion site 2) a constitutive promoter (PCON) 3) the ORF of the trans-acting replication factor (Trans) 4) a terminator 5) a kanamycin resistance cassette (KnR) flanked by FRT sites and 6) a ~500 bp HA matching the 3' end of the genomic insertion site. Full sequences can be found in Additional file 1, Table S1.

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