Table 2 Oligos used for library construction
From: Rapid optimization of gene dosage in E. coli using DIAL strains
pir | pBjk2741-jtk3041 | 5'-3' oligos |
|---|---|---|
| Â | Outer oligo F | CACATGGCGACCAGATCAATAC |
| Â | Outer oligo R | ATTGCCGCAGGTGGAAAC |
| Â | Inner oligo F | GGTACAGTGCTAGCGGATCTNNNGGAANNNNNNRTGAGACTCAAGGTCATGATGG |
| Â | Inner oligo R | GATCCGCTAGCACTGTACC |
repA | pBjk2648r-jtk2951 | 5'-3' oligos |
| Â | Outer oligo F | GGATTTTCCTTGTTTCCAGAG |
| Â | Outer oligo R | GCTTACGGCTTTATATTACGGG |
| Â | Inner oligo F | CTCGTCAGTAACGAGCCCTNNNAGAANNNNNNRTGAGCGCCGTACTTC |
| Â | Inner oligo R | AGGGCTCGTTACTGACGAG |