Plasmid design and nomenclature of BglBrick plasmids (pBb). (A) Plasmid design of pBb vectors. The plasmid is composed of three modules: antibiotic resistance gene module, replication origin module, and expression module, which includes the repressor, promoter, gene of interest (rfp or gfp), and terminator. BglBrick sites are in red boxes. (B) Nomenclature of the pBb vector system. The identity of the vector is described by three letters containing the information of replication origin, promoter, and antibiotic resistance marker as indicated. The prefix pBb is used for BglBrick plasmids and the protein gene name in the plasmid is included at the end of the vector description.