BglBrick plasmid copy numbers in DH1 and BLR(DE3). The blue bars are for DH1 strains, and the purple bars are for BLR(DE3) strains. The dark colored bars are for the single-plasmid strain, and the light colored bars are for the three-plasmid strain containing pBbA8a-CFP, pBbE5c-YFP and pBbS2k-RFP. Plasmids with the pBBR1 origin were not tested in the three-plasmid strain. (A) Plasmid copy number for single plasmid strain (B) DH1 plasmid copy number comparison for single and three plasmid strain (C) BLR(DE3) plasmid copy number comparison for single and three plasmid strain.