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Table 2 Primers for BglBrick vector construction

From: BglBrick vectors and datasheets: A synthetic biology platform for gene expression

Name Primers for promoter system Description
pSC101QC F1 5'- gaatttacagatacccagatcAcccgggaaaagg-3' to remove BglII site on pSC101
pSC101QC R1 5'- ccttttcccgggTgatctgggtatctgtaaattc-3' to remove BglII site on pSC101
pBBR1 F1 5'- gatcaCCTAGGctacagccgatagtctggaacagcgc -3' for pBBR1 origin with AvrII
pBBR1 mut R1 5'- ccggcaccgtgtTggcctacgtggtc -3' to increase copy number of pBBR1
pBBR1 mut F1 5'- gaccacgtaggccAacacggtgccgg -3' to increase copy number of pBBR1
pBBR1 R2 5'- agatcaACTAGTgcctccggcctgcggcctgcgcgcttcg -3' for pBBR1 origin with SpeI
CmQC F1 5'-ctttcattgccatacgAaattccggatgagcattc-3' to remove EcoRI site on CmR
CmQC R1 5'-gaatgctcatccggaattTcgtatggcaatgaaag-3' to remove EcoRI site on CmR
KanQC F1 5'- cctgtctcttgatcagatcAtgatcccctgc-3' to remove BglII site on KmR
KanQC R1 5'- gcaggggatcaTgatctgatcaagagacagg-3' to remove BglII site on KmR
RFP F1 5'- aaaAGATCTtttaagaaggagatatacatATGgcgagtagcgaagacgttatc-3' for rfp with BglII
RFP R1 5'- CTCGAGtttGGATCCttaagcaccggtggagtgacg-3' for rfp with BamHI and XhoI
Term F1 5'- gtgcttaaGGATCCaaaCTCGAGtaaggatctccaggcatcaaataaaacg-3' for double terminator with BamHI and XhoI
Term R1 5'- gatcaCCTAGGtataaacgcagaaaggcccacccgaagg -3' for double terminator with AvrII
pTrc F1 5'- agatcaGACGTCgacaccatcgaatggtgcaaaacc-3' for Ptrc with AatII
placUV5 R1 5'- tatctccttcttaaaAGATCTtttGAATTCtgaaattgttatccgctcacaattc-3' for Ptrc, PlacUV5, and PT7 with EcoRI and BglII
pTet F1 5'- agatcaGACGTCttaagacccactttcacatttaagttg-3' for Ptet with AatII
pTet R1 5'- tatctccttcttaaaAGATCTtttGAATTCttttctctatcactgatagggagtgg-3' for Ptet with EcoRI and BglII
pProS F1 5'- agatcaGACGTCttaattacccgactggtctttggcac -3' for Salmonela based PprpB with AatII
pProS R2 5'- gggatatcagcctggaattTgatcatctggcgacc -3' to remove EcoRI site
pProS F2 5'- ggtcgccagatgatcAaattccaggctgatatccc -3' to remove EcoRI site
pProS R1 5'- tatctccttcttaaaAGATCTtttGAATTCcatgttagtaaattgttattcaag -3' for Salmonela based PprpB with EcoRI and BglII
pProE F1 5'- agatcaGACGTCtcagcttttcagccgccgccagaac -3' for E. coli based PprpB with AatII
pProE R2 5'- gtttcgcgatatcagcctTgagtttgatcacctgg -3' to remove XhoI site
pProE F2 5'- ccaggtgatcaaactcAaggctgatatcgcgaaac -3' to remove XhoI site
pProE R1 5'- tatctccttcttaaaAGATCTtttGAATTCttgttatcaacttgttatttgcgttg -3' for E. coli based PprpB with EcoRI and BglII
lacUV5 F1 5'- agatcaGACGTCggtgcctaatgagtgagctaacttacattaattgc-3' for PlacUV5 with AatII
PLlacO-1 F1 5'- agatcaGACGTCggtgcctaatgagtgagctaacttacattaattg-3' for lacI with AatII
PLlacO-1 R2 5'- aatgtcaattgttatccgctcacaattctcgatcctctacgccggacg-3' for lacI
PLlacO-1 F2 5'- cgtccggcgtagaggatcgagaattgtgagcggataacaattgacatt-3' for PLlacO-1
PLlacO-1 R1 5'- tatctccttcttaaaAGATCTtttGAATTCggtcagtgcgtcctgctgatgtg-3' for PLlacO-1 with EcoRI and BglII
pT7 F1 5'- agatcaGACGTCctcactgcccgctttccagtc-3' for PT7 with AatII
pBAD F1 5'- agatcaGACGTCttatgacaacttgacggctacatcattcac-3' for PBAD with AatII
pBAD R2 5'- gataaaaagcgtcaggtagAatccgctaatcttatgg-3' to remove BamHI site
pBAD F2 5'-ccataagattagcggatTctacctgacgctttttatc-3' to remove BamHI site
pBAD R1 5'-tatctccttcttaaaAGATCTtttGAATTCccaaaaaaacgggtatggagaaacag-3' for PBAD with EcoRI and BglII