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Table 2 Primers for BglBrick vector construction

From: BglBrick vectors and datasheets: A synthetic biology platform for gene expression

Name

Primers for promoter system

Description

pSC101QC F1

5'- gaatttacagatacccagatcAcccgggaaaagg-3'

to remove BglII site on pSC101

pSC101QC R1

5'- ccttttcccgggTgatctgggtatctgtaaattc-3'

to remove BglII site on pSC101

pBBR1 F1

5'- gatcaCCTAGGctacagccgatagtctggaacagcgc -3'

for pBBR1 origin with AvrII

pBBR1 mut R1

5'- ccggcaccgtgtTggcctacgtggtc -3'

to increase copy number of pBBR1

pBBR1 mut F1

5'- gaccacgtaggccAacacggtgccgg -3'

to increase copy number of pBBR1

pBBR1 R2

5'- agatcaACTAGTgcctccggcctgcggcctgcgcgcttcg -3'

for pBBR1 origin with SpeI

CmQC F1

5'-ctttcattgccatacgAaattccggatgagcattc-3'

to remove EcoRI site on CmR

CmQC R1

5'-gaatgctcatccggaattTcgtatggcaatgaaag-3'

to remove EcoRI site on CmR

KanQC F1

5'- cctgtctcttgatcagatcAtgatcccctgc-3'

to remove BglII site on KmR

KanQC R1

5'- gcaggggatcaTgatctgatcaagagacagg-3'

to remove BglII site on KmR

RFP F1

5'- aaaAGATCTtttaagaaggagatatacatATGgcgagtagcgaagacgttatc-3'

for rfp with BglII

RFP R1

5'- CTCGAGtttGGATCCttaagcaccggtggagtgacg-3'

for rfp with BamHI and XhoI

Term F1

5'- gtgcttaaGGATCCaaaCTCGAGtaaggatctccaggcatcaaataaaacg-3'

for double terminator with BamHI and XhoI

Term R1

5'- gatcaCCTAGGtataaacgcagaaaggcccacccgaagg -3'

for double terminator with AvrII

pTrc F1

5'- agatcaGACGTCgacaccatcgaatggtgcaaaacc-3'

for Ptrc with AatII

placUV5 R1

5'- tatctccttcttaaaAGATCTtttGAATTCtgaaattgttatccgctcacaattc-3'

for Ptrc, PlacUV5, and PT7 with EcoRI and BglII

pTet F1

5'- agatcaGACGTCttaagacccactttcacatttaagttg-3'

for Ptet with AatII

pTet R1

5'- tatctccttcttaaaAGATCTtttGAATTCttttctctatcactgatagggagtgg-3'

for Ptet with EcoRI and BglII

pProS F1

5'- agatcaGACGTCttaattacccgactggtctttggcac -3'

for Salmonela based PprpB with AatII

pProS R2

5'- gggatatcagcctggaattTgatcatctggcgacc -3'

to remove EcoRI site

pProS F2

5'- ggtcgccagatgatcAaattccaggctgatatccc -3'

to remove EcoRI site

pProS R1

5'- tatctccttcttaaaAGATCTtttGAATTCcatgttagtaaattgttattcaag -3'

for Salmonela based PprpB with EcoRI and BglII

pProE F1

5'- agatcaGACGTCtcagcttttcagccgccgccagaac -3'

for E. coli based PprpB with AatII

pProE R2

5'- gtttcgcgatatcagcctTgagtttgatcacctgg -3'

to remove XhoI site

pProE F2

5'- ccaggtgatcaaactcAaggctgatatcgcgaaac -3'

to remove XhoI site

pProE R1

5'- tatctccttcttaaaAGATCTtttGAATTCttgttatcaacttgttatttgcgttg -3'

for E. coli based PprpB with EcoRI and BglII

lacUV5 F1

5'- agatcaGACGTCggtgcctaatgagtgagctaacttacattaattgc-3'

for PlacUV5 with AatII

PLlacO-1 F1

5'- agatcaGACGTCggtgcctaatgagtgagctaacttacattaattg-3'

for lacI with AatII

PLlacO-1 R2

5'- aatgtcaattgttatccgctcacaattctcgatcctctacgccggacg-3'

for lacI

PLlacO-1 F2

5'- cgtccggcgtagaggatcgagaattgtgagcggataacaattgacatt-3'

for PLlacO-1

PLlacO-1 R1

5'- tatctccttcttaaaAGATCTtttGAATTCggtcagtgcgtcctgctgatgtg-3'

for PLlacO-1 with EcoRI and BglII

pT7 F1

5'- agatcaGACGTCctcactgcccgctttccagtc-3'

for PT7 with AatII

pBAD F1

5'- agatcaGACGTCttatgacaacttgacggctacatcattcac-3'

for PBAD with AatII

pBAD R2

5'- gataaaaagcgtcaggtagAatccgctaatcttatgg-3'

to remove BamHI site

pBAD F2

5'-ccataagattagcggatTctacctgacgctttttatc-3'

to remove BamHI site

pBAD R1

5'-tatctccttcttaaaAGATCTtttGAATTCccaaaaaaacgggtatggagaaacag-3'

for PBAD with EcoRI and BglII