From: BglBrick vectors and datasheets: A synthetic biology platform for gene expression
Name | Primers for promoter system | Description |
---|---|---|
pSC101QC F1 | 5'- gaatttacagatacccagatcAcccgggaaaagg-3' | to remove BglII site on pSC101 |
pSC101QC R1 | 5'- ccttttcccgggTgatctgggtatctgtaaattc-3' | to remove BglII site on pSC101 |
pBBR1 F1 | 5'- gatcaCCTAGGctacagccgatagtctggaacagcgc -3' | for pBBR1 origin with AvrII |
pBBR1 mut R1 | 5'- ccggcaccgtgtTggcctacgtggtc -3' | to increase copy number of pBBR1 |
pBBR1 mut F1 | 5'- gaccacgtaggccAacacggtgccgg -3' | to increase copy number of pBBR1 |
pBBR1 R2 | 5'- agatcaACTAGTgcctccggcctgcggcctgcgcgcttcg -3' | for pBBR1 origin with SpeI |
CmQC F1 | 5'-ctttcattgccatacgAaattccggatgagcattc-3' | to remove EcoRI site on CmR |
CmQC R1 | 5'-gaatgctcatccggaattTcgtatggcaatgaaag-3' | to remove EcoRI site on CmR |
KanQC F1 | 5'- cctgtctcttgatcagatcAtgatcccctgc-3' | to remove BglII site on KmR |
KanQC R1 | 5'- gcaggggatcaTgatctgatcaagagacagg-3' | to remove BglII site on KmR |
RFP F1 | 5'- aaaAGATCTtttaagaaggagatatacatATGgcgagtagcgaagacgttatc-3' | for rfp with BglII |
RFP R1 | 5'- CTCGAGtttGGATCCttaagcaccggtggagtgacg-3' | for rfp with BamHI and XhoI |
Term F1 | 5'- gtgcttaaGGATCCaaaCTCGAGtaaggatctccaggcatcaaataaaacg-3' | for double terminator with BamHI and XhoI |
Term R1 | 5'- gatcaCCTAGGtataaacgcagaaaggcccacccgaagg -3' | for double terminator with AvrII |
pTrc F1 | 5'- agatcaGACGTCgacaccatcgaatggtgcaaaacc-3' | for Ptrc with AatII |
placUV5 R1 | 5'- tatctccttcttaaaAGATCTtttGAATTCtgaaattgttatccgctcacaattc-3' | for Ptrc, PlacUV5, and PT7 with EcoRI and BglII |
pTet F1 | 5'- agatcaGACGTCttaagacccactttcacatttaagttg-3' | for Ptet with AatII |
pTet R1 | 5'- tatctccttcttaaaAGATCTtttGAATTCttttctctatcactgatagggagtgg-3' | for Ptet with EcoRI and BglII |
pProS F1 | 5'- agatcaGACGTCttaattacccgactggtctttggcac -3' | for Salmonela based PprpB with AatII |
pProS R2 | 5'- gggatatcagcctggaattTgatcatctggcgacc -3' | to remove EcoRI site |
pProS F2 | 5'- ggtcgccagatgatcAaattccaggctgatatccc -3' | to remove EcoRI site |
pProS R1 | 5'- tatctccttcttaaaAGATCTtttGAATTCcatgttagtaaattgttattcaag -3' | for Salmonela based PprpB with EcoRI and BglII |
pProE F1 | 5'- agatcaGACGTCtcagcttttcagccgccgccagaac -3' | for E. coli based PprpB with AatII |
pProE R2 | 5'- gtttcgcgatatcagcctTgagtttgatcacctgg -3' | to remove XhoI site |
pProE F2 | 5'- ccaggtgatcaaactcAaggctgatatcgcgaaac -3' | to remove XhoI site |
pProE R1 | 5'- tatctccttcttaaaAGATCTtttGAATTCttgttatcaacttgttatttgcgttg -3' | for E. coli based PprpB with EcoRI and BglII |
lacUV5 F1 | 5'- agatcaGACGTCggtgcctaatgagtgagctaacttacattaattgc-3' | for PlacUV5 with AatII |
PLlacO-1 F1 | 5'- agatcaGACGTCggtgcctaatgagtgagctaacttacattaattg-3' | for lacI with AatII |
PLlacO-1 R2 | 5'- aatgtcaattgttatccgctcacaattctcgatcctctacgccggacg-3' | for lacI |
PLlacO-1 F2 | 5'- cgtccggcgtagaggatcgagaattgtgagcggataacaattgacatt-3' | for PLlacO-1 |
PLlacO-1 R1 | 5'- tatctccttcttaaaAGATCTtttGAATTCggtcagtgcgtcctgctgatgtg-3' | for PLlacO-1 with EcoRI and BglII |
pT7 F1 | 5'- agatcaGACGTCctcactgcccgctttccagtc-3' | for PT7 with AatII |
pBAD F1 | 5'- agatcaGACGTCttatgacaacttgacggctacatcattcac-3' | for PBAD with AatII |
pBAD R2 | 5'- gataaaaagcgtcaggtagAatccgctaatcttatgg-3' | to remove BamHI site |
pBAD F2 | 5'-ccataagattagcggatTctacctgacgctttttatc-3' | to remove BamHI site |
pBAD R1 | 5'-tatctccttcttaaaAGATCTtttGAATTCccaaaaaaacgggtatggagaaacag-3' | for PBAD with EcoRI and BglII |