Table 1 Effect of growth regulators on callus induction from Centella asiatica leaf explants

Blank

NIL

-

-

-

NAA

2.68

-

-

-

NAA

5.37

+

75 ± 4.2^c

Green, compact, embryogenic

NAA

10.74

++

66 ± 6.7^d

Green, compact, embryogenic

NAA

21.48

+++

100 ± 4^a

Green, compact, embryogenic

BA

2.21

-

-

-

BA

4.42

+++

66 ± 7.1^d

Green, compact, embryogenic

BA

8.84

+++

100 ± 3.1^a

Green, compact, embryogenic

BA

17.68

-

-

-

NAA+BA

2.68+2.21

+++

100 ± 3.5^a

Lush green, compact, embryogenic

NAA+BA

5.37+2.21

++

100 ± 4.6^a

Granular, light green, nonembryogenic

NAA+BA

10.74+2.21

+

40 ± 8.2^e

Brownish, very small

NAA+BA

21.48+2.21

++

92 ± 4.9^b

Compact, green, embryogenic

NAA+BA

2.68+4.42

++++

89 ± 3.7^b

Compact, green, embryogenic

NAA+BA

5.37+4.42

++++

100 ± 2.1^a

Compact, green, embryogenic

NAA+BA

10.74+4.42

++

83 ± 4^c

Friable, brownish green, non embryogenic

NAA+BA

21.48+4.42

++

88 ± 2.8^b

Friable, brownish green, non embryogenic

NAA+BA

2.68+8.84

+++

88 ± 3.3^b

Compact, whitish green

NAA+BA

5.37+8.84

++++

100 ± 2.1^a

Lush green, compact, embryogenic

NAA+BA

10.74+8.84

++++

88 ± 5^b

Lush green, compact, embryogenic

NAA+BA

21.48+8.84

+

88 ± 8^b

Compact, brownish green, embryogenic

NAA+BA

2.68+17.68

++

83 ± 7.1^c

Lush green, compact, embryogenic

NAA+BA

5.37+17.68

++++

100 ± 3.4^a

Dark green, compact, embryogenic

NAA+BA

10.74+17.68

++++

79 ± 5.7^c

Whitish green, compact, embryogenic

NAA+BA

21.48+17.68

+++

100 ± 1.9^a

Greenish white, compact, embryogenic