Detection and quantification of poliovirus infection using FTIR spectroscopy and cell culture

Table 1 Comparison of poliovirus prediction models using different infrared regions and virus titers

Method	IR region used	h.p.i	Virus titers (PFU)	Latent variables	R²	RMSEC	RMSECV
Initial	650 - 1650 cm^-1	1.5	0, 10² - 10⁴	4	0.910	0.4225	0.7093
	650 - 1650 cm^-1	4	0, 10¹, 10³, 10⁴	3	0.891	0.5225	0.8900
	650 - 1650 cm^-1	6	0, 10¹, 10², 10⁴	6	0.868	0.4914	0.8800
	650 - 1650 cm^-1	8	0 - 10⁴	7	0.911	0.4290	0.8471
Revised	650 - 3600 cm^-1	8	0 - 10⁴	7	0.819	0.6480	0.9187
	650 - 1650 cm^-1	8	0 - 10³	7	0.917	0.3298	0.5726
	iPLS 9 regions	8	0 - 10³	7	0.903	0.3577	0.5581
	iPLS 9 regions	8	0, 10² - 10⁴	7	0.944	0.4019	0.6628
	iPLS 9 regions	8	0 - 10²	7	0.964	0.1640	0.3163
	650-1650 cm^-1	12	0 - 10³	7	0.716	0.5963	1.2866

Table shows root mean square error of calibration (RMSEC), root mean square error of cross validation (RMSECV) and correlation coefficient for each model.

ISSN: 1754-1611