Table 2 Observed changes in relative peak absorbance values with corresponding biomolecules
From: Detection and quantification of poliovirus infection using FTIR spectroscopy and cell culture
Peak wavenumber (cm-1) | Corresponding biomolecules | Observed changes in cell absorbance following PV1 infection relative to uninfected control cells |
|---|---|---|
700 | (Broad) cis-C-H out-of-plane bend | General increase |
835 - 840 | C2' endo/anti (B-form helix) conformation and left handed helix DNA (Z form) | Gradual increase corresponding to increased PV1 titer |
900 - 1350 | Phosphodiester stretching bands (from absorbance due to collagen and glycogen) | Decrease; trend is less clear at higher PV1 titers |
1040 - 100 | Symmetric stretching vibration PO2 due to RNA and DNA | General increase |
1079/80 | Symmetric phosphate stretching modes or ν(PO2 −) symmetric. Phosphate stretching modes originate from the phosphodiester groups in nucleic acids. | Sharp increase in BGMK cells infected with 101 PFU/ml titer followed by a decrease in the 102 PFU/ml infected cells then a gradual height incremental with the higher titers. |
1153 | Stretching vibrations of hydrogen bonding C-OH groups | Virus titer 102 PFU/ml shows a decrease |
1236/7 | Amide III, stretching PO2−asymmetric (phosphate I) | Decrease in the peak height for the cells infected with 102 and 103 PFU/ml; higher titers shown an increase |
1312-1317 | Collagen related, amide III band components of proteins | General increase |
1455/6 | Asymmetric CH3 bending modes of the methyl groups of proteins | General decrease |
2852, 2873, 2924/5, 2956 | Lipids region (CH2 symmetric, symmetric stretching vibration of CH3 of acyl chains, stretching C-H and asymmetric stretching vibration of CH3 of acyl chains respectively) | Decrease for infection titers 102 - 103 PFU/ml |
3084 | Stretching N-H symmetric | General increase |
3293 | OH stretching (associated) | General increase; increase gradually less from 101 - 106 PFU/ml titers |