Figure 2

Standard Assembly. This figure illustrates a typical standard assembly. The plasmid containing the promoter is digested with SpeI and PstI, and the plasmid containing the coding sequence is digested with XbaI and PstI. Both of these digests are separated using agarose gel electrophoresis and the relevant parts are recovered from the gel. The gel extracts are then ligated together and the circular product is transformed into cells. Because of the necessity for gel electrophoresis, performing this same assembly using the small-sized promoter as the insert instead of the protein coding sequence would be extremely difficult.