Figure 5

Experimental Amplified Insert Assemblies. 1) The Ptet promoter is amplified from its plasmid and inserted 5' of lacZ α with RBS. The amplified fragment is 350 bp and the insert size is 50 bp. 2) The J23100 constitutive promoter is amplified from its testing device and inserted 5' of lacZ α. The amplified fragment is 1200 bp and the insert size is 40 bp. 3) The lacZ α is amplified from its plasmid and is inserted 3' of the J23100 promoter while replacing the existing coding sequence in the testing device and restoring the standard BioBrick format. The amplified fragment is 830 bp and the insert size is 530 bp. 4) The entire promoter testing device is amplified from its plasmid and inserted 3' of lacZ α creating a composite mRFP generator and testing device. The amplified fragment is 1200 bp and the insert size is 900 bp.