DTC characterization of backbone and two-device containing constructs. A. nptII copy numbers obtained from the plasmid backbone (Figure 3A) harboring the different replicons used. Each construct was tested at least in duplicate for each medium. The mean value has been reported, with the error bars denoting standard error. Values were normalized to that of the pSC101 construct, which was assigned a mean value of one. B. nptII DTC obtained from the series of plasmid backbones (Figures 3A) for LB (orange, circle) and M9 (square, blue) media. Each construct was tested at least in duplicate for each medium. C, D. nptII and cat DTCs obtained from the series of two-device plasmids (Figure 3B). The cumulative data presented were the result of two independent experiments. Constructs were tested in duplicate during each independent experiment. Three data points (and not four) are shown for the pMB1 construct because a replicate was lost during sample preparation. RNA and copy number values in panels B, C, and D were normalized to that of the pSC101 construct, with each having been assigned a mean value of one. The regression lines that minimized the sum of squared residual error are also shown, with their corresponding coefficient of determination R2.