Figure 7
From: A Metabolic Biofuel Cell: Conversion of Human Leukocyte Metabolic Activity to Electrical Currents
Release of serotonin from activated WBCs and more specifically, PBMCs. Following the microdialysis ("dial") of 5-HT (500 nM), PBS, PBMCs and activated PBMCs, 5-HT was determined using HPLC. A recovery estimate of 9% (+/- 1%) was determined for the microdialysis probe (as calculated from 5-HT standard and dial 5-HT in A). Based on this estimate, a 5-HT concentration of approximately 291 nM (+/- 34 nM) was calculated to be associated with the activation of PBMCs at a cell density of 106 cells mL-1 (B). 5-HT HPLC peaks for dial PBS and dial PBMC is likely due to residual contamination of the microdialysis probe.