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Figure 4 | Journal of Biological Engineering

Figure 4

From: Characterization of a synthetic bacterial self-destruction device for programmed cell death and for recombinant proteins release

Figure 4

Lysis dynamics of TOP10 bearing the thermoinducible lysis device in low copy plasmid grown in microplate reader. OD600 of TOP10 with pLC-T4LysHeat induced with a temperature shift from 30°C to 42°C in the microplate reader (blue line). Heat-induced pLC-T4Lys- (green line) is shown as the negative control. Induction was performed in exponential phase at OD600 = 0.3. Error bars represent the 95% confidence interval of the estimated mean. For clarity of presentation, data points shown here are resampled with a 30-minute sampling time.

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