Figure 6
Restriction analysis of pLC-T4LysHSL mutants. Plasmid DNA was digested with EcoRI and PstI. In all the screened clones two bands (vector backbone and insert) are present except in colony 1 of mutsc2, in which four bands can be observed. The expected vector backbone (~3.2 kbp) is present in all the clones, demonstrating that deletions or insertions occurred only in the insert, while all the mutated insert bands are clearly different from the unmutated culture (~2.8 kbp). As sequencing showed, colony 1 of mutsc2 had plasmids with two different mutated inserts in the same clone, one of which containing an EcoRI restriction site that, when digested, produces 2 bands.