Figure 7

Mutations occurred in the insert of pLC-T4LysHSL in mutant clones. Unmutated BBa_K173015 (A); luxR disruption mediated by IS10R (B); luxR disruption mediated by IS10R (insertion in reverse direction relative to (B)) (C); deletion of the DNA fragment flanked by BBa_B0015, containing the lux promoter and the holin and lysozyme genes (D); two different mutated plasmids in the same clone: insertion of IS10R and IS5 (containing an EcoRI restriction site) upstream of the first nucleotide of BBa_B0034 RBS and luxR, respectively (E); t gene disruption mediated by IS10R (insertion in reverse direction relative to (B)) (F). The number under the parts denotes the nucleotide of the basic part flanking the insertion sequence that has disrupted the part. The disrupted genes are luxR (BBa_C0062), encoding the transcriptional activator of lux promoter (BBa_R0062), and t (BBa_K112805), encoding the holin. Part codes are given according to the Registry of Standard Biological Parts.